秒速时时彩后一七码:技术讨论

产品咨询

1. How long will my antibody last if it is properly stored?

Our antibodies are under guarantee for 1 year after purchasing. However, internal studies show that the antibody will remain functional for a minimum of five years when properly stored.

2. How should I store my antibodies? Do I need to store it in aliquots?

Please store the antibody in the original tube at -20°C and do not aliquot. Our antibodies are stored in 50% glycerol which prevents them from freezing.

3. Could you send me detailed information for this antibody?

You can find the detailed information for each antibody on their product datasheet. Please search and click the antibody of your interest to be directed to the datasheet for your antibody which includes validated applications, images, etc. If you would like to print the datasheet, click the print icon below the Catalog Number.

4. What is the concentration of my antibody?

The concentration of a given antibody is found on the individual datasheet.

5. Can you provide me with the immunogen sequence of this antibody?

On the product page of your antibody, please click the link beside ‘Immunogen’ to view a description of the immunogen. Should you require more detailed information, please speak to a member of staff.

6. Is there a blocking peptide available for my antibody?

We use full-length recombinant proteins as immunogens to generate our antibodies that can be used as blocking reagents. However, we do not test the neutralizing effect of the antigen (the recombinant protein) and do not have any other blocking peptides against our antibodies.

7. Has my antibody been tested on any species other than human?

We test our antibodies on human and mouse cells or tissues. To find information on other species, please contact us.

8. Does my antibody cross-react with other species?

This depends on the extent of protein sequence similarity between the immunogen and the potential cross-reactive protein sequence. A pair-wise sequence alignment can be performed online through NCBI-BLAST website (Link to //blast.ncbi.nlm.nih.gov/Blast.cgi).

9. Has the antibody already been published?

Each product pages shows the number of publications and has links to the publication, if the antibody has already been cited.

10. Why is the predicted WB band size different from the predicted one?

?Several reasons can cause a difference in band size, such as multimers, gel migration, relative charges, splice variants, post-translational modification or cleavage. For more information, please contact our tech support.

11. What is the clone number?

Each monoclonal antibody has a clone number that is specific for its single clone of hybridoma cells. The clone number can be found on the product page next to the catalog number.

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融合蛋白

1. What method does Proteintech use for recombinant protein purification?

We usually add 6x His tags or GST tags to the N-terminus of proteins and these proteins are then purified by Ni2+ and glutathione affinity chromatography respectively.

2. What storage buffers are used for your recombinant protein?

The His-tagged recombinant protein is usually stored in 1x PBS buffer (58mM Na2HPO4, 17mM NaH2PO4, 68mM NaCl, pH 7.4) containing 300mM imidazole.
The GST-tagged recombinant protein is usually stored in 1x PBST buffer (58mM Na2HPO4, 17mM NaH2PO4, 68mM NaCl, 1% Triton-X100, pH 7.4) containing 100mM glutathione (GSH).

ELISA 试剂盒

What does the ELISA kit contain?

Antibody-coated 96-well microplate, assay standard, detection antibody, HRP-conjugated antibody, sample diluent, detection diluent, wash buffer, tetramethylbenzidine (TMB) substrate, stop solution, and plate cover seals.

Which ELISA type do you offer?

All Proteintech ELISA kits are sandwich ELISAs.

Are the plates pre-coated?

Yes, all plates are pre-coated with an antibody that is specific for the target.

Do I have to run the ELISA standards and samples in duplicate/triplicate?

It is recommended to run the experiment in duplicate at a minimum.

Is it possible to use the reagents of two different ELISA kits/lots?

No, different lot numbers cannot be mixed.

Are shorter or longer incubation times possible?

Incubation times should be followed exactly as stated in the manual.

How should I store the reagent?

Please follow the instructions in the manual.

Can I use a non-validated sample type with this ELISA kit?

Most of our ELISA kits are validated for cell culture supernatant, plasma, or serum.

How sensitive are the ELISA kits?

The ELISA sensitivity is highlighted in the data sheet for each kit.

Why does my standard shows very low or no color development?

There are multiple explanations: incorrect storage, incorrect incubation time, improper antibody, insufficient washing, etc. For more information, please contact technical support.

What are the reasons for high background?

Improper washing, contaminated reagents, samples, or wells. For more information, please contact technical support.

客户定制

Please visit the custom production section for information on the service we provide.?

物料安全数据表

You can download our Materials Safety Datasheets for our products here: